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Athletes and bodybuilders claim that hGH increases lean body mass and decreases the fat mass. The use of hGH in sport today is not only based on its anabolic properties, but also on its effect on carbohydrate and fat metabolism. International federations and the International Olympic Committee have had hGH on the list of forbidden compounds since 1989, when it became obvious that the development of biotechnology products based on the recombination of DNA made hGH much more easily available on the regular and black markets. In the 2006 Prohibited List, hGH in listed under class S2 of hormones and related substances. Erythropoietin (EPO) and corticotrophin as well as insulin?like growth factor (IGF)?1 and insulin also belong to the same category of peptide hormones. During the 2004 Olympics in Athens, for the first time, the so?called direct method of Wu et al 4 was used. None of the positive serum samples were finally declared positive; this was because of the too?short time window of detection of the test and the short half?life of circulating GH (about 20 minutes). Growth hormone and exercise. The effect of acute exercise on production of GH in the body has been widely described in the literature. During more intensive exercise (with accumulation of lactate at 70% V o 2 max for a short term period such as 10’20 minutes) hGH will increase by 5’10?fold. Furthermore, it appears that hGH response is more closely related to the peak intensity of exercise than the total work output. This appears evident when the training is very hard and above the aerobic threshold. Apart from exercise related increase, hGH secretion can be affected by other factors’for example, GH secretion is increased in hypoglycaemia, increased temperature, and stress, whereas it decreases in obesity, or with a carbohydrate?rich diet and intake of ? 2 adrenergic agonists. Thus, it is hard to differentiate between the physiological increase in hGH levels seen in exercise and what can be from external hGH administration (as in doping). This problem makes the purely quantitative approach of measuring directly the total circulating GH not feasible in case of doping, except if the conditions of collection of biological samples are well controlled. Effect of hGH in the body. Somatotrope cells in the anterior pituitary secrete hGH in a pulsatile fashion. The secretion is regulated by two hypothalamic peptides, growth hormone releasing hormone, which stimulates hGH secretion, and somatostatin, which inhibits hGH secretion by back regulation. Secretion of hGH is slightly higher in women than in men, 11 with the highest levels observed at puberty. Secretion decreases with age by around 14% per decade. Some drugs, such as clonidine, l ?dopa and ??hydroxybutyrate, increase its secretion, as do androgens and estrogens. Therapeutic use of hGH. Human GH is prescribed for both childhood and adulthood hGH deficiency and for girls with Turner’s syndrome. High doses of hGH are used for relief from excessive burns or other thermal injuries. Since the late 1950s, children with GH deficiency have been treated with hGH extracted from cadaver pituitary glands, gp test suspension 100 mg injectable steroids $27.00testosterone suspension.
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