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Due to technical problems, one biopsy from the Clean group was discarded, leaving 6 biopsies in the Clean group for analysis. Serial muscle cross-sections were cut at ?20’C by using a Reichert Jung cryostat (Leica, Nussloch, Germany). Eight m thick sections were stained with haematoxylin-eosin and a modified Gomori trichrome staining for basic histopathology including detection of degenerative processes and inflammation [20]., creatine important muscle growth. Five ?m thick transverse sections were processed for IHC with different and previously characterized antibodies.
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Therefore, dietary supplementation of creatine serves to increase muscle creatine and pcr by 20–40% (see fig. ) [7, 8, 10, 46,47,48]. The most effective way to increase muscle creatine stores is to ingest 5 g of creatine monohydrate (or approximately 0. 3 g/kg body weight) four times daily for 5–7 days [7, 10] Are Anabolic Steroids Addictive, creatine important muscle growth.
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